Stationary-phase optimized selectivity in supercritical fluid chromatography using a customized Phase OPtimized Liquid Chromatography kit: comparison of different prediction approaches

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Selectivity Optimization with Commercial Coupled Columns by Gradient Stationary Phase Optimized Selectivity Liquid Chromatography

In previous studies stationary phase optimized selectivity liquid chromatography (SOSLC) has been already proved as a promising technique to optimize the selectivity of a given separation [1, 2]. By combination of different stationary phases, SOSLC offers excellent possibilities for method development under both isocratic and linear gradient conditions. However, the so far available commercial ...

متن کامل

Supercritical fluid chromatography and microbore liquid chromatography for drug analysis.

Supercritical fluid and microbore liquid chromatography offer potential applications for drug analysis. In supercritical fluid chromatography (SFC), the mobile phase is a gas (e.g., carbon dioxide) maintained at its supercritical state--that is, above its critical temperature and pressure, above which it cannot be liquefied even with further increases in applied pressure. The SFC mobile phase h...

متن کامل

Optimized Nonlinear Gradients for Reversed-Phase Liquid Chromatography in Shotgun Proteomics

Reversed-phase liquid chromatography has become the preferred method for separating peptides in most of the mass spectrometry-based proteomics workflows of today. In the way the technique is typically applied, the peptides are released from the chromatography column by the gradual addition of an organic buffer according to a linear function. However, when applied to complex peptide mixtures, th...

متن کامل

An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography.

An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared for the first time by clicking arginine onto silica gel. It offers an efficient separation of organic acids, nucleotides and sugars. More interestingly, it exhibited excellent selectivity and enrichment toward acidic glycopeptides, even at a ratio of 1 : 150 to non-glycopeptides.

متن کامل

A novel amide stationary phase for hydrophilic interaction liquid chromatography and ion chromatography.

A novel amide stationary phase (ASP) for hydrophilic interaction liquid chromatography (HILIC) has been prepared via the Click chemistry method. It was based on the strategy that the amino group of Asparagine was easily transferred to the corresponding azido group and then clicked onto terminal alkyne-silica gel in the presence of Cu(I)-based catalyst. For the tested polar compounds including n...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Analytical and Bioanalytical Chemistry

سال: 2020

ISSN: 1618-2642,1618-2650

DOI: 10.1007/s00216-020-02739-w